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Sampling Guide

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ALLFLEX TSU: (See image of tools)​ *This is the preferred sampling method.

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  1. Load the Allflex tissue applicator with an unused tissue punch (red plunger is visible in used punches).

  2. Slide the applicator over the ear, and position the cutter approximately 1 inch from the edge of the ear.

  3. Squeeze the applicator handles together to take a sample, then release to free the ear.

  4. Remove the tissue punch from the device and check that the sampling has been successful.

  5. Store Allflex TSU at room temperature for up to 12 months.

  6. Ship samples at room temperature. 

 

HAIR SAMPLES:

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  1. Pull hair from between hooves with clean nose pliers and include 40 hair follicles (clear or mucous colored bulbs at the end of the hair).

  2. Place hair samples in self-sealing envelopes labeled with the sample ID and the producer or farm name.

 

SEMEN SAMPLES: 

 

  1. Remove the straw of semen from the tank and make sure it is properly labeled with a unique ID for the animal. Unique ID must match what is recorded on the sample submission form.

  2. Package the straw in a protective manner to prevent breakage during transit. Common practice is to ship in cane or to place the straw inside of a hollow pen (remove ink).

  3. Contact Ramie or Karissa for shipping address.

 

WHOLE BLOOD SAMPLES (PURPLE TOP TUBES): 

 

  1. Whole blood must be collected in the purple-topped Vacutainer tubes that contains  anticoagulant.

  2. Prior to collection of the blood sample, label the blood tube with the animal’s unique ID. This unique ID must correspond to the ID on the sample submission form.

  3. After blood collection of 1mL of blood, invert the blood tube 8-10 times to mix blood with anticoagulant.

  4. Make sure to use a new, clean needle and syringe for each animal to prevent cross contamination.

  5. Keep samples refrigerated until shipping.

  6. Contact Ramie or Karissa for shipping instructions.​

 

EAR NOTCH SAMPLES: 

 

  1. Ensure the ear notching tool is clean to prevent cross-contamination between animals.

  2. Take a proper size sample. Place sample into labeled collection bag.

  3. Check that the ear notch is free of dirt, feces, and tattoo ink.

  4. Store the collected ear notches in freezer.

  5. Contact Ramie or Karissa for shipping instructions.​

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